Sulfadiazine biodegradation by Phanerochaete chrysosporium: Mechanism and degradation product identification.
Identifieur interne : 000050 ( Main/Exploration ); précédent : 000049; suivant : 000051Sulfadiazine biodegradation by Phanerochaete chrysosporium: Mechanism and degradation product identification.
Auteurs : Ting Zhang [République populaire de Chine] ; Ling Cai [République populaire de Chine] ; Bentuo Xu [République populaire de Chine] ; Xicheng Li [République populaire de Chine] ; Wenhui Qiu [République populaire de Chine] ; Caixia Fu [République populaire de Chine] ; Chunmiao Zheng [République populaire de Chine]Source :
- Chemosphere [ 1879-1298 ] ; 2019.
Descripteurs français
- KwdFr :
- Analyse de profil d'expression de gènes (MeSH), Antibactériens (métabolisme), Assainissement et restauration de l'environnement (méthodes), Concentration en ions d'hydrogène (MeSH), Dépollution biologique de l'environnement (MeSH), Facteurs temps (MeSH), Peroxidases (métabolisme), Phanerochaete (génétique), Phanerochaete (métabolisme), Polluants environnementaux (métabolisme), Protéines fongiques (métabolisme), Spectrométrie de masse en tandem (MeSH), Sulfadiazine (métabolisme).
- MESH :
- génétique : Phanerochaete.
- métabolisme : Antibactériens, Peroxidases, Phanerochaete, Polluants environnementaux, Protéines fongiques, Sulfadiazine.
- méthodes : Assainissement et restauration de l'environnement.
- Analyse de profil d'expression de gènes, Concentration en ions d'hydrogène, Dépollution biologique de l'environnement, Facteurs temps, Spectrométrie de masse en tandem.
English descriptors
- KwdEn :
- Anti-Bacterial Agents (metabolism), Biodegradation, Environmental (MeSH), Environmental Pollutants (metabolism), Environmental Restoration and Remediation (methods), Fungal Proteins (metabolism), Gene Expression Profiling (MeSH), Hydrogen-Ion Concentration (MeSH), Peroxidases (metabolism), Phanerochaete (genetics), Phanerochaete (metabolism), Sulfadiazine (metabolism), Tandem Mass Spectrometry (MeSH), Time Factors (MeSH).
- MESH :
- chemical , metabolism : Anti-Bacterial Agents, Environmental Pollutants, Fungal Proteins, Peroxidases, Sulfadiazine.
- genetics : Phanerochaete.
- metabolism : Phanerochaete.
- methods : Environmental Restoration and Remediation.
- Biodegradation, Environmental, Gene Expression Profiling, Hydrogen-Ion Concentration, Tandem Mass Spectrometry, Time Factors.
Abstract
Antibiotic contaminants have become a severe environmental problem in recent years and finding effective ways to deal with this issue is of great importance. In this study, Phanerochaete chrysosporium was used to degrade sulfadiazine (SDZ), which is frequently detected in the culture medium of isolates from soil and surface water systems. The results demonstrate that 10 mg L-1 SDZ can be completely degraded by P. chrysosporium under conditions of pH 5.7 and 30 °C within 6 days. The Q-Exactive-MS/MS analysis identified and confirmed several different SDZ degradation intermediates, and four proposed degradation pathways of SDZ were deduced. Moreover, enzyme activity tests revealed that manganese peroxidase and ligninolytic peroxidase played important roles in SDZ degradation. Moreover, a transcriptome analysis method was performed to explore the mechanism and pathways of SDZ degradation by P. chrysosporium in greater detail. The results of GO and KEGG analysis strongly suggest that the metabolism pathway is significantly activated and plays an important role in antibiotic degradation. Further, this is the first study to identify SDZ degradation intermediates and two main intermediates were found to be involved in possible SDZ degradation pathways. This study is also the first report results from RNA sequencing to evaluate genome-wide changes of P. chrysosporium to further explore SDZ degradation mechanism.
DOI: 10.1016/j.chemosphere.2019.124418
PubMed: 31369901
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Sulfadiazine biodegradation by Phanerochaete chrysosporium: Mechanism and degradation product identification.</title><author><name sortKey="Zhang, Ting" sort="Zhang, Ting" uniqKey="Zhang T" first="Ting" last="Zhang">Ting Zhang</name><affiliation wicri:level="1"><nlm:affiliation>Guangdong Provincial Key Laboratory of Soil and Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen, 518055, China; State Environmental Protection Key Laboratory of Integrated Surface Water-Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen, 518055, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Guangdong Provincial Key Laboratory of Soil and Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen, 518055, China; State Environmental Protection Key Laboratory of Integrated Surface Water-Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen, 518055</wicri:regionArea><wicri:noRegion>518055</wicri:noRegion></affiliation></author><author><name sortKey="Cai, Ling" sort="Cai, Ling" uniqKey="Cai L" first="Ling" last="Cai">Ling Cai</name><affiliation wicri:level="1"><nlm:affiliation>Third Institute of Oceanography, Ministry of Natural Resources, PR China, Xiamen, 361005, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Third Institute of Oceanography, Ministry of Natural Resources, PR China, Xiamen, 361005</wicri:regionArea><wicri:noRegion>361005</wicri:noRegion></affiliation></author><author><name sortKey="Xu, Bentuo" sort="Xu, Bentuo" uniqKey="Xu B" first="Bentuo" last="Xu">Bentuo Xu</name><affiliation wicri:level="1"><nlm:affiliation>College of Life and Environmental Science, Wenzhou University, Wenzhou, 325035, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life and Environmental Science, Wenzhou University, Wenzhou, 325035</wicri:regionArea><wicri:noRegion>325035</wicri:noRegion></affiliation></author><author><name sortKey="Li, Xicheng" sort="Li, Xicheng" uniqKey="Li X" first="Xicheng" last="Li">Xicheng Li</name><affiliation wicri:level="1"><nlm:affiliation>Guangdong Provincial Key Laboratory of Soil and Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen, 518055, China; State Environmental Protection Key Laboratory of Integrated Surface Water-Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen, 518055, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Guangdong Provincial Key Laboratory of Soil and Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen, 518055, China; State Environmental Protection Key Laboratory of Integrated Surface Water-Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen, 518055</wicri:regionArea><wicri:noRegion>518055</wicri:noRegion></affiliation></author><author><name sortKey="Qiu, Wenhui" sort="Qiu, Wenhui" uniqKey="Qiu W" first="Wenhui" last="Qiu">Wenhui Qiu</name><affiliation wicri:level="1"><nlm:affiliation>Guangdong Provincial Key Laboratory of Soil and Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen, 518055, China; State Environmental Protection Key Laboratory of Integrated Surface Water-Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen, 518055, China. Electronic address: qiuwh@sustech.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Guangdong Provincial Key Laboratory of Soil and Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen, 518055, China; State Environmental Protection Key Laboratory of Integrated Surface Water-Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen, 518055</wicri:regionArea><wicri:noRegion>518055</wicri:noRegion></affiliation></author><author><name sortKey="Fu, Caixia" sort="Fu, Caixia" uniqKey="Fu C" first="Caixia" last="Fu">Caixia Fu</name><affiliation wicri:level="1"><nlm:affiliation>Guangdong Provincial Key Laboratory of Soil and Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen, 518055, China; State Environmental Protection Key Laboratory of Integrated Surface Water-Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen, 518055, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Guangdong Provincial Key Laboratory of Soil and Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen, 518055, China; State Environmental Protection Key Laboratory of Integrated Surface Water-Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen, 518055</wicri:regionArea><wicri:noRegion>518055</wicri:noRegion></affiliation></author><author><name sortKey="Zheng, Chunmiao" sort="Zheng, Chunmiao" uniqKey="Zheng C" first="Chunmiao" last="Zheng">Chunmiao Zheng</name><affiliation wicri:level="1"><nlm:affiliation>Guangdong Provincial Key Laboratory of Soil and Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen, 518055, China; State Environmental Protection Key Laboratory of Integrated Surface Water-Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen, 518055, China. Electronic address: zhengcm@sustech.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Guangdong Provincial Key Laboratory of Soil and Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen, 518055, China; State Environmental Protection Key Laboratory of Integrated Surface Water-Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen, 518055</wicri:regionArea><wicri:noRegion>518055</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2019">2019</date><idno type="RBID">pubmed:31369901</idno><idno type="pmid">31369901</idno><idno type="doi">10.1016/j.chemosphere.2019.124418</idno><idno type="wicri:Area/Main/Corpus">000054</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000054</idno><idno type="wicri:Area/Main/Curation">000054</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000054</idno><idno type="wicri:Area/Main/Exploration">000054</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Sulfadiazine biodegradation by Phanerochaete chrysosporium: Mechanism and degradation product identification.</title><author><name sortKey="Zhang, Ting" sort="Zhang, Ting" uniqKey="Zhang T" first="Ting" last="Zhang">Ting Zhang</name><affiliation wicri:level="1"><nlm:affiliation>Guangdong Provincial Key Laboratory of Soil and Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen, 518055, China; State Environmental Protection Key Laboratory of Integrated Surface Water-Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen, 518055, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Guangdong Provincial Key Laboratory of Soil and Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen, 518055, China; State Environmental Protection Key Laboratory of Integrated Surface Water-Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen, 518055</wicri:regionArea><wicri:noRegion>518055</wicri:noRegion></affiliation></author><author><name sortKey="Cai, Ling" sort="Cai, Ling" uniqKey="Cai L" first="Ling" last="Cai">Ling Cai</name><affiliation wicri:level="1"><nlm:affiliation>Third Institute of Oceanography, Ministry of Natural Resources, PR China, Xiamen, 361005, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Third Institute of Oceanography, Ministry of Natural Resources, PR China, Xiamen, 361005</wicri:regionArea><wicri:noRegion>361005</wicri:noRegion></affiliation></author><author><name sortKey="Xu, Bentuo" sort="Xu, Bentuo" uniqKey="Xu B" first="Bentuo" last="Xu">Bentuo Xu</name><affiliation wicri:level="1"><nlm:affiliation>College of Life and Environmental Science, Wenzhou University, Wenzhou, 325035, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Life and Environmental Science, Wenzhou University, Wenzhou, 325035</wicri:regionArea><wicri:noRegion>325035</wicri:noRegion></affiliation></author><author><name sortKey="Li, Xicheng" sort="Li, Xicheng" uniqKey="Li X" first="Xicheng" last="Li">Xicheng Li</name><affiliation wicri:level="1"><nlm:affiliation>Guangdong Provincial Key Laboratory of Soil and Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen, 518055, China; State Environmental Protection Key Laboratory of Integrated Surface Water-Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen, 518055, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Guangdong Provincial Key Laboratory of Soil and Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen, 518055, China; State Environmental Protection Key Laboratory of Integrated Surface Water-Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen, 518055</wicri:regionArea><wicri:noRegion>518055</wicri:noRegion></affiliation></author><author><name sortKey="Qiu, Wenhui" sort="Qiu, Wenhui" uniqKey="Qiu W" first="Wenhui" last="Qiu">Wenhui Qiu</name><affiliation wicri:level="1"><nlm:affiliation>Guangdong Provincial Key Laboratory of Soil and Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen, 518055, China; State Environmental Protection Key Laboratory of Integrated Surface Water-Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen, 518055, China. Electronic address: qiuwh@sustech.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Guangdong Provincial Key Laboratory of Soil and Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen, 518055, China; State Environmental Protection Key Laboratory of Integrated Surface Water-Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen, 518055</wicri:regionArea><wicri:noRegion>518055</wicri:noRegion></affiliation></author><author><name sortKey="Fu, Caixia" sort="Fu, Caixia" uniqKey="Fu C" first="Caixia" last="Fu">Caixia Fu</name><affiliation wicri:level="1"><nlm:affiliation>Guangdong Provincial Key Laboratory of Soil and Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen, 518055, China; State Environmental Protection Key Laboratory of Integrated Surface Water-Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen, 518055, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Guangdong Provincial Key Laboratory of Soil and Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen, 518055, China; State Environmental Protection Key Laboratory of Integrated Surface Water-Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen, 518055</wicri:regionArea><wicri:noRegion>518055</wicri:noRegion></affiliation></author><author><name sortKey="Zheng, Chunmiao" sort="Zheng, Chunmiao" uniqKey="Zheng C" first="Chunmiao" last="Zheng">Chunmiao Zheng</name><affiliation wicri:level="1"><nlm:affiliation>Guangdong Provincial Key Laboratory of Soil and Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen, 518055, China; State Environmental Protection Key Laboratory of Integrated Surface Water-Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen, 518055, China. Electronic address: zhengcm@sustech.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Guangdong Provincial Key Laboratory of Soil and Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen, 518055, China; State Environmental Protection Key Laboratory of Integrated Surface Water-Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen, 518055</wicri:regionArea><wicri:noRegion>518055</wicri:noRegion></affiliation></author></analytic><series><title level="j">Chemosphere</title><idno type="eISSN">1879-1298</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Anti-Bacterial Agents (metabolism)</term><term>Biodegradation, Environmental (MeSH)</term><term>Environmental Pollutants (metabolism)</term><term>Environmental Restoration and Remediation (methods)</term><term>Fungal Proteins (metabolism)</term><term>Gene Expression Profiling (MeSH)</term><term>Hydrogen-Ion Concentration (MeSH)</term><term>Peroxidases (metabolism)</term><term>Phanerochaete (genetics)</term><term>Phanerochaete (metabolism)</term><term>Sulfadiazine (metabolism)</term><term>Tandem Mass Spectrometry (MeSH)</term><term>Time Factors (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de profil d'expression de gènes (MeSH)</term><term>Antibactériens (métabolisme)</term><term>Assainissement et restauration de l'environnement (méthodes)</term><term>Concentration en ions d'hydrogène (MeSH)</term><term>Dépollution biologique de l'environnement (MeSH)</term><term>Facteurs temps (MeSH)</term><term>Peroxidases (métabolisme)</term><term>Phanerochaete (génétique)</term><term>Phanerochaete (métabolisme)</term><term>Polluants environnementaux (métabolisme)</term><term>Protéines fongiques (métabolisme)</term><term>Spectrométrie de masse en tandem (MeSH)</term><term>Sulfadiazine (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Anti-Bacterial Agents</term><term>Environmental Pollutants</term><term>Fungal Proteins</term><term>Peroxidases</term><term>Sulfadiazine</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Environmental Restoration and Remediation</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Antibactériens</term><term>Peroxidases</term><term>Phanerochaete</term><term>Polluants environnementaux</term><term>Protéines fongiques</term><term>Sulfadiazine</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Assainissement et restauration de l'environnement</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Biodegradation, Environmental</term><term>Gene Expression Profiling</term><term>Hydrogen-Ion Concentration</term><term>Tandem Mass Spectrometry</term><term>Time Factors</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de profil d'expression de gènes</term><term>Concentration en ions d'hydrogène</term><term>Dépollution biologique de l'environnement</term><term>Facteurs temps</term><term>Spectrométrie de masse en tandem</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Antibiotic contaminants have become a severe environmental problem in recent years and finding effective ways to deal with this issue is of great importance. In this study, Phanerochaete chrysosporium was used to degrade sulfadiazine (SDZ), which is frequently detected in the culture medium of isolates from soil and surface water systems. The results demonstrate that 10 mg L<sup>-1</sup> SDZ can be completely degraded by P. chrysosporium under conditions of pH 5.7 and 30 °C within 6 days. The Q-Exactive-MS/MS analysis identified and confirmed several different SDZ degradation intermediates, and four proposed degradation pathways of SDZ were deduced. Moreover, enzyme activity tests revealed that manganese peroxidase and ligninolytic peroxidase played important roles in SDZ degradation. Moreover, a transcriptome analysis method was performed to explore the mechanism and pathways of SDZ degradation by P. chrysosporium in greater detail. The results of GO and KEGG analysis strongly suggest that the metabolism pathway is significantly activated and plays an important role in antibiotic degradation. Further, this is the first study to identify SDZ degradation intermediates and two main intermediates were found to be involved in possible SDZ degradation pathways. This study is also the first report results from RNA sequencing to evaluate genome-wide changes of P. chrysosporium to further explore SDZ degradation mechanism.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" IndexingMethod="Curated" Owner="NLM"><PMID Version="1">31369901</PMID><DateCompleted><Year>2019</Year><Month>12</Month><Day>30</Day></DateCompleted><DateRevised><Year>2019</Year><Month>12</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1879-1298</ISSN><JournalIssue CitedMedium="Internet"><Volume>237</Volume><PubDate><Year>2019</Year><Month>Dec</Month></PubDate></JournalIssue><Title>Chemosphere</Title><ISOAbbreviation>Chemosphere</ISOAbbreviation></Journal><ArticleTitle>Sulfadiazine biodegradation by Phanerochaete chrysosporium: Mechanism and degradation product identification.</ArticleTitle><Pagination><MedlinePgn>124418</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S0045-6535(19)31639-X</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.chemosphere.2019.124418</ELocationID><Abstract><AbstractText>Antibiotic contaminants have become a severe environmental problem in recent years and finding effective ways to deal with this issue is of great importance. In this study, Phanerochaete chrysosporium was used to degrade sulfadiazine (SDZ), which is frequently detected in the culture medium of isolates from soil and surface water systems. The results demonstrate that 10 mg L<sup>-1</sup> SDZ can be completely degraded by P. chrysosporium under conditions of pH 5.7 and 30 °C within 6 days. The Q-Exactive-MS/MS analysis identified and confirmed several different SDZ degradation intermediates, and four proposed degradation pathways of SDZ were deduced. Moreover, enzyme activity tests revealed that manganese peroxidase and ligninolytic peroxidase played important roles in SDZ degradation. Moreover, a transcriptome analysis method was performed to explore the mechanism and pathways of SDZ degradation by P. chrysosporium in greater detail. The results of GO and KEGG analysis strongly suggest that the metabolism pathway is significantly activated and plays an important role in antibiotic degradation. Further, this is the first study to identify SDZ degradation intermediates and two main intermediates were found to be involved in possible SDZ degradation pathways. This study is also the first report results from RNA sequencing to evaluate genome-wide changes of P. chrysosporium to further explore SDZ degradation mechanism.</AbstractText><CopyrightInformation>Copyright © 2019. Published by Elsevier Ltd.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Ting</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Guangdong Provincial Key Laboratory of Soil and Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen, 518055, China; State Environmental Protection Key Laboratory of Integrated Surface Water-Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen, 518055, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cai</LastName><ForeName>Ling</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Third Institute of Oceanography, Ministry of Natural Resources, PR China, Xiamen, 361005, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Bentuo</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>College of Life and Environmental Science, Wenzhou University, Wenzhou, 325035, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Xicheng</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Guangdong Provincial Key Laboratory of Soil and Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen, 518055, China; State Environmental Protection Key Laboratory of Integrated Surface Water-Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen, 518055, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Qiu</LastName><ForeName>Wenhui</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>Guangdong Provincial Key Laboratory of Soil and Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen, 518055, China; State Environmental Protection Key Laboratory of Integrated Surface Water-Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen, 518055, China. Electronic address: qiuwh@sustech.edu.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Fu</LastName><ForeName>Caixia</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Guangdong Provincial Key Laboratory of Soil and Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen, 518055, China; State Environmental Protection Key Laboratory of Integrated Surface Water-Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen, 518055, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zheng</LastName><ForeName>Chunmiao</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Guangdong Provincial Key Laboratory of Soil and Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen, 518055, China; State Environmental Protection Key Laboratory of Integrated Surface Water-Groundwater Pollution Control, School of Environmental Science and Engineering, Southern University of Science and Technology, Shenzhen, 518055, China. Electronic address: zhengcm@sustech.edu.cn.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>07</Month><Day>25</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Chemosphere</MedlineTA><NlmUniqueID>0320657</NlmUniqueID><ISSNLinking>0045-6535</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000900">Anti-Bacterial Agents</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004785">Environmental Pollutants</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005656">Fungal Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0N7609K889</RegistryNumber><NameOfSubstance UI="D013411">Sulfadiazine</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.11.1.-</RegistryNumber><NameOfSubstance UI="D010544">Peroxidases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.11.1.13</RegistryNumber><NameOfSubstance UI="C051129">manganese peroxidase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000900" MajorTopicYN="N">Anti-Bacterial Agents</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001673" MajorTopicYN="N">Biodegradation, Environmental</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004785" MajorTopicYN="N">Environmental Pollutants</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D052918" MajorTopicYN="N">Environmental Restoration and Remediation</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005656" MajorTopicYN="N">Fungal Proteins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020869" MajorTopicYN="N">Gene Expression Profiling</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006863" MajorTopicYN="N">Hydrogen-Ion Concentration</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010544" MajorTopicYN="N">Peroxidases</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020075" MajorTopicYN="N">Phanerochaete</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013411" MajorTopicYN="N">Sulfadiazine</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D053719" MajorTopicYN="N">Tandem Mass Spectrometry</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013997" MajorTopicYN="N">Time Factors</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Antibiotics</Keyword><Keyword MajorTopicYN="N">Biodegradation</Keyword><Keyword MajorTopicYN="N">RNA sequencing</Keyword><Keyword MajorTopicYN="N">Sulfadiazine</Keyword><Keyword MajorTopicYN="N">Transcription analysis</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>05</Month><Day>11</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2019</Year><Month>07</Month><Day>09</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>07</Month><Day>19</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>8</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>12</Month><Day>31</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>8</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">31369901</ArticleId><ArticleId IdType="pii">S0045-6535(19)31639-X</ArticleId><ArticleId IdType="doi">10.1016/j.chemosphere.2019.124418</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Zhang, Ting" sort="Zhang, Ting" uniqKey="Zhang T" first="Ting" last="Zhang">Ting Zhang</name></noRegion><name sortKey="Cai, Ling" sort="Cai, Ling" uniqKey="Cai L" first="Ling" last="Cai">Ling Cai</name><name sortKey="Fu, Caixia" sort="Fu, Caixia" uniqKey="Fu C" first="Caixia" last="Fu">Caixia Fu</name><name sortKey="Li, Xicheng" sort="Li, Xicheng" uniqKey="Li X" first="Xicheng" last="Li">Xicheng Li</name><name sortKey="Qiu, Wenhui" sort="Qiu, Wenhui" uniqKey="Qiu W" first="Wenhui" last="Qiu">Wenhui Qiu</name><name sortKey="Xu, Bentuo" sort="Xu, Bentuo" uniqKey="Xu B" first="Bentuo" last="Xu">Bentuo Xu</name><name sortKey="Zheng, Chunmiao" sort="Zheng, Chunmiao" uniqKey="Zheng C" first="Chunmiao" last="Zheng">Chunmiao Zheng</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PhanerochaeteV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000050 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000050 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PhanerochaeteV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:31369901
|texte= Sulfadiazine biodegradation by Phanerochaete chrysosporium: Mechanism and degradation product identification.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:31369901" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PhanerochaeteV1
| This area was generated with Dilib version V0.6.37. |  |